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Kleihauer-Betke Hemoglobin F Acid Resistance

View larger version (157K): [in this window] [in a new window]   Figure 1. Hemoglobin F in RBCs is resistant to acid elution. Cells containing hemoglobin F stain bright red with eosin, while those without hemoglobin F appear as colorless ghosts.

View larger version (179K): [in this window] [in a new window]   Figure 2. RBCs of a normal adult appear colorless after RBC acid elution and eosin counterstaining.

View larger version (170K): [in this window] [in a new window]   Figure 3. This blood smear is from a postpartum patient who had a significant fetal-maternal hemorrhage during delivery. The fetal RBCs with high hemoglobin F are bright red while the maternal RBCs show either faint staining or are colorless.

View larger version (175K): [in this window] [in a new window]   Figure 4. Blood smear form a patient with beta thalassemia shows bright colored cells, intermediate staining cells and colorless cells reflecting the uneven distribution of hemoglobin F among the RBCs. In another thalassemia, hereditary persistence of fetal hemoglobin, all RBCs will stain bright red.

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This Article Figures Only Services Similar articles in this journal Alert me to new content in the Image Bank Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Lazarchick, J. Search for Related Content PubMed Articles by Lazarchick, J. Related Collections Hemolytic Disorders Related ASH-SAP Chapter Related Image Bank Image Sets Related ASH Education Book Articles Social Bookmarking                   What's this?