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This Article Figures Only Services Similar articles in this journal Alert me to new content in the Image Bank Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Lazarchick, J. Search for Related Content PubMed Articles by Lazarchick, J. Related Collections Plasmapheresis Effect of G-CSF, GM-CSF Stem cell/Bone marrow Related ASH-SAP Chapter Related Image Bank Image Set Related ASH Education Book Articles Social Bookmarking                   What's this?

Pheresis collection post G-CSF therapy

View larger version (56K): [in this window] [in a new window]   Figure 1. A Wright stained smear prepared from a pheresis stem cell collection bag is shown. The donor had received prednisone and G-CSF prior to the collection. Immature mononuclear cells, most with granular cytoplasm, are present. The pheresis cell collection is then analyzed by flow cytometric analysis to determine CD34 content.

View larger version (52K): [in this window] [in a new window]   Figure 2. Myeloid cells at all stages of differentiation (blast to band forms) are shown in this image. Of note is the hypergranularity of the cytoplasm in the more differentiated forms. The granules have a distinct violaceous hue that is characteristic of G-CSF therapy. In addition, there are immature forms present that have the morphologic appearance suggesting monocytic lineage with cytoplasmic vacuolization and irregular nuclear borders.

View larger version (77K): [in this window] [in a new window]   Figure 3. A higher magnification of the previous image is shown illustrating in detail the characteristic morphologic features of the myeloid response following G-CSF stem cell mobilization.

View larger version (125K): [in this window] [in a new window]   Figure 4. A dual esterase histochemical stain (chloroacetate esterase for myeloid cell and butyrate esterase for monocytoid cells) of a blood smear made from the collection bag is shown. Myleoid lineage cells have a magenta color while monocytoid lineage cells stain black. The predominant cells type is myeloid, however, monocytoid cells are also present.

View larger version (109K): [in this window] [in a new window]   Figure 5. Magenta staining myeloid cells at all stages of maturation are shown. Platelets and the two lymphocytes in this view show no stain.

View larger version (111K): [in this window] [in a new window]   Figure 6. A single cell of monocytic lineage (black stain - arrow) is shown in this view.

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This Article Figures Only Services Similar articles in this journal Alert me to new content in the Image Bank Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Lazarchick, J. Search for Related Content PubMed Articles by Lazarchick, J. Related Collections Plasmapheresis Effect of G-CSF, GM-CSF Stem cell/Bone marrow Related ASH-SAP Chapter Related Image Bank Image Set Related ASH Education Book Articles Social Bookmarking                   What's this?